Dr. Stefan Hell and Leica Microsystems are pioneers in the discovery of smallness,
things less than two
hundredths of a hair's breadth. A normal light microscope can no longer make
these visible, it is a physical limit. That
it is possible to exceed these limits and show processes at the nanoscale in
high resolution was shown by physicist Stefan Hell. For this achievement he is receiving the
Nobel Prize. These achievements also
contains quite a bit of Leica and Wetzlar.
Stefan Walter Hell
For 141 years the law of
microscopy by pioneer Ernst Abbe (1840-1905) is in effect. It defines that the resolution
limit of a microscope depends on the wavelength of light: Anything smaller than
200 nanometers can no longer be optically visualized in a recognizable resolution,
it is totally out of focus.
Nobel Prize winner Stefan
Hell has found a way to circumvent the law. He employed fluorescence: A laser
pulse can illuminate "colored" molecules under a microscope, a second
annular laser pulse cuts off the fluorescence in the outer region of the first
light spot, so that the inner light spot is smaller than 200 nanometers. The process is called STED (Stimulated
Emission Depletion) microscopy. It
enables monitoring of processes in the nanometer range in living cells. With
the Leica STED microscope resolutions of up to 50 nm are possible.
Wetzlar is the German city of
optics. Already in the mid-19th
century a lot of pioneering work had been done here, and today is no
different. The Nobel Prize in Chemistry
awarded to Stefan Hell is in honor of the technology developed by him, which
makes it possible to do basic biological research at the nanoscale. The STED microscopes were developed by Leica
Microsystems in Wetzlar. "We were
the pioneers in the commercial super-resolution microscopy," says Dr.
Tanjef Szellas, Head of light microscopy.
When it was announced that Hell
is receiving the Nobel Prize for his discovery, the employees of Leica
Microsystems and the professor in Göttingen were elated. Many know him
personally. Employees of Leica
Microsystems and Hells research group worked together for a long time, some even
switched over to Leica Microsystems.
Hell’s discovery isn’t
totally new. Already in the 90s he filed for a patent for the STED microscopy. Leica Microsystems acquired the exclusive
license for the use of the STED-patent in the 90s and worked with Hell on
implementing the technology.
Already in 2004 Leica
Microsystems introduced the first super-resolution microscope, a predecessor
technology of the STED method. Two years
later they received the Innovationspreis (Innovation Award) of the German
industry. 2007 followed the first STED
microscope. The term "microscope" no longer corresponds to what many
know from their biology classes. Rather,
it is a complex high-tech system, as Szellas explained. A STED system could
fill a small room, there are laser units, and the microscope is remotely
controlled via software and requires a vibration-damped table. Instead of the eyepiece, the scientists look
at monitors; the microscope is connected to a computer. Scientists can thus see, for example, exactly
how a virus enters a cell in the body; the basis for the study of the emergence
and treatment of diseases.
Leica TCS SP8 STED 3X
150 STED systems have been
sold and delivered by Leica Microsystems since 2007, each costing an average
600,000 euros. These high-tech microscopes are used, for example, at the Freie
Universität Berlin (Free University Berlin) and the University in Göttingen, where
research is done to discover which processes occur in nerve cells. The findings help to understand how learning
and memory functions in the brain.
------------------------------------------------------------------------------------------------------------------------------------------
Order: info@gmpphoto.com
Please make payment
via PayPal to GMP Photography
Click on ad to
enlarge
Order: info@gmpphoto.com
Please make payment
via PayPal to GMP Photography
Click on ad to
enlarge
Order: info@gmpphoto.com
Please make payment
via PayPal to GMP Photography
What does this have to do with photography?
ReplyDeleteWell, the Leica STED microscope could be considered to be a special lens which makes it possible to photograph extremely small items, like living cells.
ReplyDeleteSince Leica is the first part in the name of this blog, and since Leica Microsystems are part of the four companies carrying the Leica name, I thought this might be an interesting contribution to this blog. Leica Microsystems also owns the rights to the Leica name. Leica Camera AG is using via a licensing agreement.